Improved Protocol for Isolation of Genomic DNA from Leaf Tissues of Phyllanthus emblica Gaertn
Authors: not saved
Abstract:
Modified Cetyltrimethylammonium bromide (CTAB) protocol for DNA isolation was developed from leaf tissuesof Phyllanthus emblica for obtaining high quality genomic DNA. Fresh leaves of three different maturitywere analyzed for yield and quality of DNA. Acidity was determined in three different maturity of leaves viz.tender, intermediate and mature and their influence on DNA quality was determined. Drastic reduction of pHwas the primary cause for poor quality of DNA. However, high quality DNA isolation was achieved bystabilizing the pH by addition of NaOH during different stages of DNA isolation process. The present protocolyielding high quality intact DNA for genetic fingerprinting as well as for amplification of chloroplast genes formolecular analysis.
similar resources
Isolation and Characterization of Polyphenolic Compound Quercitin from Phyllanthus Emblica
The curative parts of medicinal plants are not simply its woody stem or its leaves but the number of chemical compounds (phytoconstituents) it produces and uses for its own growth like flavonoids, alkaloids, gum, mucilage, protein, carbohydrate etc. The genus Phyllanthus is widely used in medicine but still lot of work left to explore this genus. So the species, Phyllanthus emblica which is wid...
full textProtocol for extraction of genomic DNA from swine solid tissues
Molecular diagnostics are performed by using DNA from different body tissues. However, it is necessary to obtain genomic DNA of good quality. Due to the impossibility of collecting blood from slaughtered animals, DNA extraction from solid tissues is necessary. The objective of this study was to describe a protocol of DNA extraction from swine skin, adipose, brain, liver, kidney and muscle tissu...
full textCorrelation between scavenging property and antioxidant activity in the extracts of Emblica officinalis Gaertn., syn. Phyllanthus emblica L. Fruit
The present study investigates a concentration-dependent assessment of scavenging property and antioxidant activity in the extracts of Emblica officinalis Gaertn. syn. Phyllanthus emblica L. fruit using various in vitro assays. A correlation of aqueous and ethanolic extract of Emblica fruit along with different known standards for its antioxidative properties was made by performing assays like ...
full textAn Efficient Protocol for Genomic Dna Isolation from Cultivable Bacteria
A standardized protocol can play a major role in isolation of DNA especially when dealing with bacterial cultures. The aim of the present study was to develop a standard protocol of ultrapure high yield bacterial genomic DNA extraction from bacterial culture suitable for use in molecular biology. Extraction procedure was optimized with series of steps, which involved an unpublished modification...
full textComparison study of three methods for genomic DNA extraction from fresh and herbarium leaf specimens of Achillea wilhelmsii C.Koch
DNA extraction from plant tissues often causes most problems. For example, unsuccessful removal secondary metabolites during extraction, such as phenolic compounds in aromatic and medicinal plants, cause to some mistakes in result of molecular experiments by using of the extracted DNA. Achillea wilhelmsii is a medicinal plant belong to Asteraceae family and native to Iran, there is little infor...
full textAnticancer Properties of Phyllanthus emblica (Indian Gooseberry)
There is a wealth of information emanating from both in vitro and in vivo studies indicating fruit extract of the Phyllanthus emblica tree, commonly referred to as Indian Gooseberries, has potent anticancer properties. The bioactivity in this extract is thought to be principally mediated by polyphenols, especially tannins and flavonoids. It remains unclear how polyphenols from Phyllanthus embli...
full textMy Resources
Journal title
volume 9 issue 4
pages 307- 313
publication date 2011-10-01
By following a journal you will be notified via email when a new issue of this journal is published.
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023